کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6142237 | 1594351 | 2015 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functions of the 5â²- and 3â²-untranslated regions of tobamovirus RNA
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
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چکیده انگلیسی
The tobamovirus genome is a 5â²-m7G-capped RNA that carries a tRNA-like structure at its 3â²-terminus. The genomic RNA serves as the template for both translation and negative-strand RNA synthesis. The 5â²- and 3â²-untranslated regions (UTRs) of the genomic RNA contain elements that enhance translation, and the 3â²-UTR also contains the elements necessary for the initiation of negative-strand RNA synthesis. Recent studies using a cell-free viral RNA translation-replication system revealed that a 70-nucleotide region containing a part of the 5â²-UTR is bound cotranslationally by tobacco mosaic virus (TMV) replication proteins translated from the genomic RNA and that the binding leads the genomic RNA to RNA replication pathway. This mechanism explains the cis-preferential replication of TMV by the replication proteins. The binding also inhibits further translation to avoid a fatal ribosome-RNA polymerase collision, which might arise if translation and negative-strand synthesis occur simultaneously on a single genomic RNA molecule. Therefore, the 5â²- and 3â²-UTRs play multiple important roles in the life cycle of tobamovirus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 206, 3 August 2015, Pages 82-89
Journal: Virus Research - Volume 206, 3 August 2015, Pages 82-89
نویسندگان
Tetsuya Chujo, Kazuhiro Ishibashi, Shuhei Miyashita, Masayuki Ishikawa,