کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6156234 | 1247939 | 2013 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Clinical significance of microRNA-148a in patients with early relapse of stage II stage and III colorectal cancer after curative resection
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کلمات کلیدی
RT-qPCRDFScDNA - cDNAComplementary DNA - DNA تکمیلیdisease-free survival - بقاء بدون بیماریoverall survival - بقای کلColorectal cancer - سرطان روده بزرگconfidence interval - فاصله اطمینانMicro-RNA - میکرو RNAMiRNA - میکروRNA، ریزآرانای، miRNAodds ratio - نسبت شانس هاpolymerase chain reaction - واکنش زنجیره ای پلیمرازReverse Transcription-quantitative Polymerase Chain Reaction - واکنش زنجیره ای پلیمراز کمی رونویسی معکوسPCR - واکنش زنجیرهٔ پلیمرازCRC - کد افزونگی دورهای
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
پزشکی و دندانپزشکی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Development of robust prognostic/predictive biomarkers in patients with colorectal cancer (CRC) is imperative for advancing treatment strategies for this disease. We aimed to determine whether expression status of microRNAs might be a simple and reliable biomarker to detect postoperative early relapse in patients with CRC after radical resection. We used microRNA arrays and identified that microRNA-148a (miRNA-148a) had substantially different expression levels in early and nonearly relapsed stage II and III CRC tissues. The validation study, which included 55 early relapsed patients and 55 nonearly relapsed patients, further confirmed overexpression of miRNA-148a in nonearly relapsed samples. Subsequently, we explored whether the serum level of miRNA-148a can be used to predict early CRC recurrence. The in vitro and in vivo effects of miRNA-148a were examined by cell proliferation, migration, and invasion, as well as cell cycles, and xenograft in null mice. Last, miRNA-148a was investigated as a potential biomarker for identifying early relapse. Cellular studies demonstrated that the overexpression of miRNA-148a inhibited colon cancer cell proliferation and migration but not invasion. The cell cycle studies also revealed that miRNA-148a caused an accumulation of the G2 population. Moreover, lower levels of miRNA-148a expression were associated with significantly shorter disease-free survival rates and poorer overall survival rates. This study showed that miRNA-148a can inhibit tumorigenesis and reduce the early recurrence of CRC. These findings suggest that miRNA-148a may have potential clinical applications for predicting the early relapse of patients with CRC after radical resection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Translational Research - Volume 162, Issue 4, October 2013, Pages 258-268
Journal: Translational Research - Volume 162, Issue 4, October 2013, Pages 258-268
نویسندگان
Hsiang-Lin Tsai, I-Ping Yang, Ching-Wen Huang, Cheng-Jen Ma, Chao-Hung Kuo, Chien-Yu Lu, Suh-Hang Juo, Jaw-Yuan Wang,