کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6226789 1276404 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Utility of Scalp Hair Follicles as a Novel Source of Biomarker Genes for Psychiatric Illnesses
ترجمه فارسی عنوان
استفاده از فولیکول های موی پوستی به عنوان منبع جدید ژن های بیومارکر برای بیماری های روانپزشکی
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی روانپزشکی بیولوژیکی
چکیده انگلیسی

BackgroundIdentifying beneficial surrogate genetic markers in psychiatric disorders is crucial but challenging.MethodsGiven that scalp hair follicles are easily accessible and, like the brain, are derived from the ectoderm, expressions of messenger RNA (mRNA) and microRNA in the organ were examined between schizophrenia (n for first/second = 52/42) and control subjects (n = 62/55) in two sets of cohort. Genes of significance were also analyzed using postmortem brains (n for case/control = 35/35 in Brodmann area 46, 20/20 in cornu ammonis 1) and induced pluripotent stem cells (n = 4/4) and pluripotent stem cell-derived neurospheres (n = 12/12) to see their role in the central nervous system. Expression levels of mRNA for autism (n for case/control = 18/24) were also examined using scalp hair follicles.ResultsAmong mRNA examined, FABP4 was downregulated in schizophrenia subjects by two independent sample sets. Receiver operating characteristic curve analysis determined that the sensitivity and specificity were 71.8% and 66.7%, respectively. FABP4 was expressed from the stage of neurosphere. Additionally, microarray-based microRNA analysis showed a trend of increased expression of hsa-miR-4449 (p = .0634) in hair follicles from schizophrenia. hsa-miR-4449 expression was increased in Brodmann area 46 from schizophrenia (p = .0007). Finally, we tested the expression of nine putative autism candidate genes in hair follicles and found decreased CNTNAP2 expression in the autism cohort.ConclusionsScalp hair follicles could be a beneficial genetic biomarker resource for brain diseases, and further studies of FABP4 are merited in schizophrenia pathogenesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biological Psychiatry - Volume 78, Issue 2, 15 July 2015, Pages 116-125
نویسندگان
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