کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6326504 | 1619755 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Analysis of chromosome damage by sister chromatid exchange (SCE) and redox homeostasis characterization on sheep flocks from Sardinian pasturelands
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کلمات کلیدی
LPO5-BrdUN-Tyr5-bromodeoxyuridineTACGPXASCSCETOCROS - ROSAscorbic acid - آسکوربیک اسیدAlpha-tocopherol - آلفا توکوفرولEnvironmental pollution - آلودگی زیست محیطیenzyme linked immunosorbent assay - آنزیم تست ایمونوسیورسانس مرتبط استSister chromatid exchange - تبادل کروماتید خواهرsister chromatid exchanges - تبادل کروماتید خواهرELISA - تست الیزاRET - حقRetinol - رتینولSOD - سدSuperoxide dismutase - سوکسوکس دیسموتازChromosome fragility - شکنندگی کروموزومTotal antioxidant capacity - ظرفیت آنتی اکسیدانی کلNitro-tyrosine - نیترو تیروزینRedox homeostasis - هومئوستاز ردوکسLipid hydroperoxide - هیدروپراکسید لیپیدBio-monitoring - پیگیری بیولوژیکhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کاراglutathione peroxidase - گلوتاتیون پراکسیدازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Over the last decades, an increase of pollutants of diverse origin (industrial, military, mining, etc.) was recorded in several areas of Sardinia Island. We report the results of a multidisciplinary and complementary study based on cytogenetic and physiological analyses. The data obtained show the effects of the environmental impact on six sheep flocks (Sardinian breed) grazing on natural pasturelands next to possible polluted areas and compared to three herds grazing in different areas far from those potentially contaminated and used as control. Sister chromatid exchange (SCE) test was used as cytogenetic test to analyze chromosomal damages and it was performed on peripheral blood samples collected from 129 adult sheep (age > 4 years) randomly selected from polluted (92 animals) and control (37 animals) areas. Two types of cell cultures were performed: without (normal cultures) and with the addition of 5-BrdU. SCE-mean values estimated over 35 cells counted for each animal were 8.65 ± 3.40, 8.10 ± 3.50, 8.05 ± 3.08, 7.42 ± 3.34, 9.28 ± 3.56 and 8.38 ± 3.29 in the exposed areas, whereas the average values were 7.86 ± 3.31 in the control group. Significant increases (P < 0.01) of SCEs were found in three investigated areas of Southern Sardinia. Furthermore, sheep of the same flocks were characterized for blood redox homeostasis in order to define the potential targets of oxidative damage and to identify biomarkers of the extent of animal exposure to environmental contaminants. The plasma levels of Asc, Toc and Ret were found to be significantly lower (P < 0.001) in exposed sheep (I, II, IV and V) than in the control group. TAC as well as GPx and SOD activities were higher in control than in the exposed groups (P < 0.001). Finally, plasma levels of N-Tyr, PC, and LPO were significantly lower (P < 0.001) in the control group than in the exposed groups.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Science of The Total Environment - Volumes 527â528, 15 September 2015, Pages 393-400
Journal: Science of The Total Environment - Volumes 527â528, 15 September 2015, Pages 393-400
نویسندگان
Viviana Genualdo, Angela Perucatti, Alfredo Pauciullo, Alessandra Iannuzzi, Domenico Incarnato, Maria Stefania Spagnuolo, Nicolina Solinas, Simonetta Bullitta, Leopoldo Iannuzzi,