کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6357744 1622742 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Antibiotic resistance of Vibrio harveyi isolated from seawater in Korea
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات اقیانوس شناسی
پیش نمایش صفحه اول مقاله
Antibiotic resistance of Vibrio harveyi isolated from seawater in Korea
چکیده انگلیسی
Vibrio harveyi is an opportunistic human pathogen that may cause gastroenteritis, severe necrotizing soft-tissue infections, and primary septicemia, with a potentially high rate of lethality. In this study, we isolated and characterized V. harveyi from seawater collected from the West Sea in Korea, including sites located near shellfish farms. For the initial isolation of putative V. harveyi, isolates were incubated on thiosulfate citrate bile salt sucrose agar plates for 24 h, followed by selection of greenish colonies. Gram-negative and oxidase-positive colonies were subsequently confirmed by biochemical assays and the API 20E kit test system. Species-specific 16S rRNA and hemolysin genes were used to design V. harveyi-specific PCR primers. From 840 seawater samples, a total of 2 strains of V. harveyi were isolated from shellfish farm seawater. The two isolates were subjected to profiling against 16 antibiotics and found to be resistant to cephalothin, vancomycin, ampicillin, cefepime, cefotetan, and streptomycin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Marine Pollution Bulletin - Volume 86, Issues 1–2, 15 September 2014, Pages 261-265
نویسندگان
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