کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6358255 1622740 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Monitoring toxic Ostreopsis cf. ovata in recreational waters using a qPCR based assay
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات اقیانوس شناسی
پیش نمایش صفحه اول مقاله
Monitoring toxic Ostreopsis cf. ovata in recreational waters using a qPCR based assay
چکیده انگلیسی


- Toxic Ostreopsis blooms affect Mediterranean coastal water quality.
- We applied qPCR assay to monitor and quantify Ostreopsis abundance in seawater.
- The quantification was done by site-specific environmental standard curves.
- The method could be applied in monitoring activities to control recreational water quality.

Ostreopsis sp. is a toxic marine benthic dinoflagellate that causes high biomass blooms, posing a threat to human health, marine biota and aquaculture activities, and negatively impacting coastal seawater quality. Species-specific identification and enumeration is fundamental because it can allow the implementation of all the necessary preventive measures to properly manage Ostreopsis spp. bloom events in recreational waters and aquaculture farms. The aim of this study was to apply a rapid and sensitive qPCR method to quantify Ostreopsis cf. ovata abundance in environmental samples collected from Mediterranean coastal sites and to develop site-specific environmental standard curves. Similar PCR efficiencies of plasmid and environmental standard curves allowed us to estimate the LSU rDNA copy number per cell. Moreover, we assessed the effectiveness of mitochondrial COI and cob genes as alternative molecular markers to ribosomal genes in qPCR assays for Ostreopsis spp. quantification.

80

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Marine Pollution Bulletin - Volume 88, Issues 1–2, 15 November 2014, Pages 102-109
نویسندگان
, , , , , , ,