کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6358468 1315630 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Acute effects of heavy metals on the expression of glutathione-related antioxidant genes in the marine ciliate Euplotes crassus
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات اقیانوس شناسی
پیش نمایش صفحه اول مقاله
Acute effects of heavy metals on the expression of glutathione-related antioxidant genes in the marine ciliate Euplotes crassus
چکیده انگلیسی
Euplotes crassus, a single-celled eukaryote, is directly affected by environmental contaminants. Here, exponentially cultured E. crassus were exposed to cadmium, copper, lead, and zinc and then the reactive oxygen species (ROS) and total glutathione (GSH) levels were measured. Subsequently, the transcriptional modulation of glutathione peroxidase (GPx) and glutathione reductase (GR) were estimated by quantitative RT-PCR. After an 8-h exposure, significantly higher increases in the relative ROS and total GSH levels were observed in exposed group, compared to the controls. Real-time PCR data revealed that the expression levels of GPx and GR mRNA were sensitively modulated within 8 h of exposure to all heavy metals. These findings suggest that these genes may be involved in cellular defense mechanisms by modulating their gene expression against heavy metal-induced oxidative stress. Thus, they may be useful as potential molecular biomarkers to assess sediment environments for contaminants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Marine Pollution Bulletin - Volume 85, Issue 2, 30 August 2014, Pages 455-462
نویسندگان
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