کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6359820 1315643 2013 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An integrated microfluidic device in marine microalgae culture for toxicity screening application
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات اقیانوس شناسی
پیش نمایش صفحه اول مقاله
An integrated microfluidic device in marine microalgae culture for toxicity screening application
چکیده انگلیسی


- We develop a microfluidic device based on microalgal culture for toxicity testing.
- We validated and modeled marine microalgal culture in microfluidics.
- Using the device, marine microalgae were successfully stressed on-chip.
- A single assay can provided on-lined multi biological response measurements.

Algal assay using marine microalgae has emerged as an important method to evaluate the toxicity of chemicals, which is currently undertaken using conventional culture and additional detection of physiological cellular endpoints. While effective, this approach can be labor-intensive and thus could benefit from a more streamlined, integrated approach. Microfluidics offers a way to accomplish this goal. Here, we demonstrate a microfluidic device which consists of a concentration gradient generator (CGG), diffusible culturing module and power-free valve system. It allows the processes of chemical liquid dilution and diffusion, micro-scale microalgal culture (in batch or chemostatic conditions), cell stimulation and on-lined screening to be integrated into a single device. Using the device, marine microalgae were successfully cultured and stressed on-chip. The simple assay provides multi-biological response measurements of cell division rate, autofluorescence and esterase activity. This work showed promising in developing a microfluidic platform for toxicity screening based on marine microalgal culture.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Marine Pollution Bulletin - Volume 72, Issue 1, 15 July 2013, Pages 231-243
نویسندگان
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