Spatial configuration of extracellular polymeric substances of Bacillus megaterium TF10 in aqueous solution

► Spatial configuration of microbial EPS is characterized using laser light scattering technique. ► EPS exist in the form of colloids in aqueous solution. ► Cryo-electron microscopy image confirms the spherical shape of EPS colloids. ► LLS approach is powerful to characterize EPS configuration.