کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6382292 | 1625946 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A primary fish gill cell culture model to assess pharmaceutical uptake and efflux: Evidence for passive and facilitated transport
ترجمه فارسی عنوان
یک مدل کشت سلولی ژله ماهی برای ارزیابی میزان جذب و خروجی دارو: شواهد برای حمل و نقل منفعل و تسهیل شده
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کلمات کلیدی
CETABio-concentrationQSARsTEPBCFdpmABCMRPpKa3RsSLCOECDApical - اپیکالOct - اکتبرBtA - بتاdisintegrations per minute - تخریب در دقیقهAnimal alternatives - جایگزین های حیوانیsolute carrier - حامل رقیبDissociation constant - حد تفکیکorganic cation transporter - حمل و نقل کاتیونی آلیPharmaceuticals - داروهاTER - داشتنREACH - رسیدنQuantitative structure–activity relationships - روابط فعالیت ساختاری کمیOrganisation for Economic Co-operation and Development - سازمان همکاری اقتصادی و توسعهapparent permeability coefficient - ضریب نفوذپذیری ظاهریbioconcentration factor - فاکتور زیستی کنسانترهRainbow trout - قزل آلای رنگین کمانFish - ماهیTransepithelial resistance - مقاومت TransepithelialPapp - پاپbasal - پایهTransepithelial potential - پتانسیل Transepithelialmultidrug resistance protein - پروتئین مقاوم در برابر چندین رژیمcurie - کوریKow - کوهATP-binding cassette - کیت اتصال به ATP
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
چکیده انگلیسی
The gill is the principle site of xenobiotic transfer to and from the aqueous environment. To replace, refine or reduce (3Rs) the large numbers of fish used in in vivo uptake studies an effective in vitro screen is required that mimics the function of the teleost gill. This study uses a rainbow trout (Oncorhynchus mykiss) primary gill cell culture system grown on permeable inserts, which tolerates apical freshwater thus mimicking the intact organ, to assess the uptake and efflux of pharmaceuticals across the gill. Bidirectional transport studies in media of seven pharmaceuticals (propranolol, metoprolol, atenolol, formoterol, terbutaline, ranitidine and imipramine) showed they were transported transcellularly across the epithelium. However, studies conducted in water showed enhanced uptake of propranolol, ranitidine and imipramine. Concentration-equilibrated conditions without a concentration gradient suggested that a proportion of the uptake of propranolol and imipramine is via a carrier-mediated process. Further study using propranolol showed that its transport is pH-dependent and at very low environmentally relevant concentrations (ng Lâ1), transport deviated from linearity. At higher concentrations, passive uptake dominated. Known inhibitors of drug transport proteins; cimetidine, MK571, cyclosporine A and quinidine inhibited propranolol uptake, whilst amantadine and verapamil were without effect. Together this suggests the involvement of specific members of SLC and ABC drug transporter families in pharmaceutical transport.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquatic Toxicology - Volume 159, February 2015, Pages 127-137
Journal: Aquatic Toxicology - Volume 159, February 2015, Pages 127-137
نویسندگان
Lucy C. Stott, Sabine Schnell, Christer Hogstrand, Stewart F. Owen, Nic R. Bury,