کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6387949 1627592 2013 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression of three novel cytochrome P450 (CYP) and antioxidative genes from the polychaete, Perinereis nuntia exposed to water accommodated fraction (WAF) of Iranian crude oil and Benzo[α]pyrene
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات اقیانوس شناسی
پیش نمایش صفحه اول مقاله
Expression of three novel cytochrome P450 (CYP) and antioxidative genes from the polychaete, Perinereis nuntia exposed to water accommodated fraction (WAF) of Iranian crude oil and Benzo[α]pyrene
چکیده انگلیسی


- Three new cytochrome P450 (CYP) genes were cloned in the polychaete Perinereis nuntia.
- P. nuntia CYP431A1 is involved in the detoxification of the water accommodated fraction of crude oil.
- CYP3 clan gene also plays an important role in B[α]P metabolisms.
- Phase II and antioxidant enzymes were induced following PAH exposure in P. nuntia.
- P. nuntia has a potential to be used as indicator for WAF and B[α]P pollution in sediments.

To report a novel CYP genes and to evaluate its potency as a biomarker for oil pollution, we cloned three CYP genes and measured their expression profiles under controlled lab conditions using real-time reverse transcription PCR (real-time RT-PCR) after exposure of the water accommodated fraction (WAF) of Iranian crude oil and benzo[α]pyrene (B[α]P) as a positive control. Of these, CYP432A1 (CYP3 clan) gene was significantly induced by B[α]P exposure, indicating that the CYP3 clan gene would play an important role in polycyclic aromatic hydrocarbon (PAH) metabolisms, particularly for B[α]P in this species. However, the Perinereis nuntia CYP431A1 mRNA, a CYP2 clan gene, was sensitively expressed to WAF exposure with other two CYP genes. As one of Phase II detoxification enzymes, the glutathione S-transferase (GST) genes also upregulated with other antioxidant genes (SOD and CAT), indicating that WAF-exposed P. nuntia was properly responding to this kind of chemical stress. Thus, three CYP genes from the polychaete, P. nuntia have a potential as a biomarker in monitoring of the marine sediment after an oil spill accident.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Marine Environmental Research - Volume 90, September 2013, Pages 75-84
نویسندگان
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