کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6390719 1628405 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development and validation of an enzyme linked immunosorbent assay for fluoroquinolones in animal feeds
ترجمه فارسی عنوان
توسعه و اعتبار سنجی آنزیم تست ایمونوسیورسانس برای فلوروکینولون ها در خوراک حیوانات
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی


- A quick screening method for fluoroquinolone in feeds based on ELISA is validated.
- Matrix effect is avoided by only 10 fold dilution of the final feed extract.
- The achieved CCβ of 20 ng·g−1 for enrofloxacin indicates sufficient sensitivity.
- Good correlation of the method with LC-Fl indicates promising reliability.

An enzyme-linked immunosorbent assay (ELISA) for analysis of fluoroquinolones residues in animal feeds has been developed and validated according to Commission Decision 2002/657/EC criteria. Initially, direct and indirect competitive ELISA formats were compared for one fluoroquinolone polyclonal antibody (As172) and two monoclonal antibodies (FQ8 and FQ10), in order to find the best combination in terms of simplicity, reduction of matrix effect and sensitivity. The optimal methodology was identified as direct ELISA format using polyclonal antibody As172, able to avoid the matrix effect by only 10-fold dilution of feed samples. Following the optimized ELISA protocol, the half-maximal inhibitory concentration (IC50) and limit of detection (LOD) for enrofloxacin was determined to be 15.2 ng·g−1 and 1.3 ng·g−1, respectively. Decision limit (CCα) obtained was 10 ng·g−1 and detection capability (CCβ) was 20 ng·g−1. Significant cross-reactivity values (>42%) were obtained for eight fluoroquinolones by the optimized ELISA method. Moreover, comparison of results from ELISA to that of liquid chromatography with fluorescence detection (LC-Fl) showed good correlation. In general, the developed ELISA allows a rapid, sensitive, and low-cost screening analysis of fluoroquinolone residues in animal feeds.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 57, November 2015, Pages 195-201
نویسندگان
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