Generation of anti-zearalenone scFv and its incorporation into surface plasmon resonance-based assay for the detection of zearalenone in sorghum

- Generation of an anti-zearalenone scFv.
- Direct immobilization zearalenone onto CM5 sensor chip.
- Development of competitive enzyme-linked and SPR-based inhibition assays.
- Validation of SPR-based inhibition assay using sorghum reference sample.

Recombinant scFvs to zearalenone were isolated by phage display, expressed in Escherichia coli and the analytical sensitivity of selected scFvs determined by competitive inhibition enzyme-linked immunoassay (CI-ELISA) and surface plasmon resonance (SPR)-based inhibition assay. The results demonstrated higher sensitivity for the SPR-based assay compared to CI-ELISA. The applicability of the SPR-based assay for the detection of zearalenone in food samples was evaluated using sorghum.