Prevalence and characterisation of potentially virulent Vibrio parahaemolyticus in seafood in Malaysia using conventional methods, PCR and REP-PCR

► gyrB/pntA-based mPCR detected 1.5 × 103 CFU/ml Vibrio parahaemolyticus in spiked seafood. ► V. parahaemolyticus harbouring tdh and/or trh genes discriminated by REP-PCR. ► A high genetic diversity among the V. parahaemolyticus isolates.