کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6393193 | 1330449 | 2012 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Determination of aflatoxins and ochratoxin A in retail cereal products from Turkey by high performance liquid chromatography with fluorescence detection Determination of aflatoxins and ochratoxin A in retail cereal products from Turkey by high performance liquid chromatography with fluorescence detection](/preview/png/6393193.png)
In this study, a total of 110 retail cereal products from Turkey were analysed for aflatoxins (AFB1, AFB2, AFG1 and AFG2) and ochratoxin A (OTA). The mycotoxins were determined by liquid-solid extraction, immunoaffinity column clean-up and high performance liquid chromatography with fluorescence detection (HPLC-FD). Recoveries (83.9-92%) and both intra-day and inter-day repeatability (RSD < 12) of the method, meet the performance criteria set by EC regulation No. 401/2006 for the determination of the levels of mycotoxins in foodstuffs. OTA was the most prevalent toxin, with an incidence of 43.6% (range = 0.066-1.125 μg kgâ1), but at levels below the European legislation limit of 3 μg kgâ1. Aflatoxins were found in 27 of 110 analysed samples: twenty-seven samples with AFB1, fourteen samples with AFB2, seven samples with AFG1 and two samples with AFG2. The ranges for positive samples were 0.052-0.233 μg AFB1 kgâ1, 0.022-0.044 μg AFB2 kgâ1, 0.053-0.149 μg AFG1 kgâ1 and 0.033-0.037 μg AFG2 kgâ1. The co-occurrence of AFB1 and OTA was observed in 14.6% of the samples. This is the first study concerning the simultaneous occurrence of aflatoxins and OTA in retail cereal products from Turkey.
⺠AFs and OTA levels in retail cereal products from Turkey were surveyed. ⺠Samples were analysed by IAC clean-up followed by HPLC-FD detection. ⺠OTA was the most frequently detected mycotoxin. ⺠None of the samples exceeded the maximum limits set by EU for AFs and OTA. ⺠The co-occurrence of AFB1 and OTA was found in 14.6% of the samples.
Journal: Food Control - Volume 28, Issue 1, November 2012, Pages 1-6