کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6452111 1417004 2016 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Purification, enzymatic activity and inhibitor discovery for recombinant human carbonic anhydrase XIV
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Purification, enzymatic activity and inhibitor discovery for recombinant human carbonic anhydrase XIV
چکیده انگلیسی


- Recombinant preparation of CA XIV, a difficult to produce protein, is described.
- Large amount of CA XIV was affinity purified using sulfonamide-modified sepharose.
- Inhibitor titration of activity demonstrated nearly 100% pure and active enzyme.
- Nanomolar inhibitors of CA XIV, selective over CA I and II, were designed.

Human carbonic anhydrase XIV (CA XIV), a transmembrane protein, highly expressed in the central nervous system, is difficult to recombinantly express and purify in large scale for the measurements of inhibitor binding and drug design. CA XIV belongs to the family of twelve catalytically active CA isoforms in the human body. Disorders in the expression of CA XIV cause serious diseases and CA XIV has been described as a possible drug target for the treatment of epilepsy, some retinopathies, and skin tumors. In this study, the effect of different promoters, E. coli strains, and the length of recombinant CA XIV protein construct were analyzed for the production CA XIV in large scale by using affinity purification. Active site titration by inhibitors and the isothermal titration calorimery revealed over 96% purity of the protein. Enzymatic activity of the purified CA XIV was determined by following the CO2 hydration using the stopped-flow technique. Several inhibitors were discovered that exhibited selectivity towards CA XIV over other CA isoforms and could be developed as drugs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 240, 20 December 2016, Pages 31-42
نویسندگان
, , , , , , ,