کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6490835 | 43387 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Secretory production of antimicrobial peptides in Escherichia coli using the catalytic domain of a cellulase as fusion partner
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Secretory production of antimicrobial peptides in Escherichia coli using the catalytic domain of a cellulase as fusion partner Secretory production of antimicrobial peptides in Escherichia coli using the catalytic domain of a cellulase as fusion partner](/preview/png/6490835.png)
چکیده انگلیسی
Antimicrobial peptides (AMPs) are small molecules which serve as essential components of the innate immune system in various organisms. AMPs possess a broad spectrum of antimicrobial activities. However, the scaled production of such peptides in Escherichia coli faces many difficulties because of their small size and toxicity to the host. Here, we described a new fusion strategy to extracellularly produce significant amounts of these antimicrobial peptides in recombinant E. coli at significant amount. Employing the catalytic domain of a cellulase (Cel-CD) from Bacillus subtilis KSM-64 as the fusion partner, five recombinant antimicrobial peptides were confirmed to accumulate in the culture medium at concentrations ranging from 184Â mg/L to 297Â mg/L. The radical diffusion experiment demonstrated that the released model antimicrobial peptide, bombinin, had antibacterial activities against both E. coli and Staphylococcus aureus. This strategy will be suitable for the production of antimicrobial peptides and other toxicity proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 214, 20 November 2015, Pages 77-82
Journal: Journal of Biotechnology - Volume 214, 20 November 2015, Pages 77-82
نویسندگان
Huili Yu, Haoran Li, Dongfang Gao, Cuijuan Gao, Qingsheng Qi,