کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6490901 | 43386 | 2015 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enhanced protein production by microRNA-30 family in CHO cells is mediated by the modulation of the ubiquitin pathway
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کلمات کلیدی
miR-30UPLSkp2S-phase kinase-associated protein 2ncRNAUniversal Probe LibrarypDNAqRT-PCRPDLIM5CgrMMUcDNA - cDNAComplementary DNA - DNA تکمیلیNon-coding RNA - RNA غیرکد کننده، RNA غیرترجمه شوندهsmall-interfering RNA - RNA کوچک تداخل داردsiRNA - siRNAZinc finger - انگشت رویCho - برایChinese Hamster Ovary - تخمدان هامستر چینیRISC - خطرRNA-Induced Silencing Complex - مجتمع خاموش کننده RNA inducedUbiquitin pathway - مسیر اوبیکیتینCell engineering - مهندسی سلولMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNAMus musculus - نام علمی انسانPlasmid DNA - پلاسمید دی ان ای
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Enhanced protein production by microRNA-30 family in CHO cells is mediated by the modulation of the ubiquitin pathway Enhanced protein production by microRNA-30 family in CHO cells is mediated by the modulation of the ubiquitin pathway](/preview/png/6490901.png)
چکیده انگلیسی
Functional genomics represent a valuable approach to improve culture performance of Chinese hamster ovary (CHO) cell lines for biopharmaceutical manufacturing. Recent advances in applied microRNA (miRNAs) research suggest that these small non-coding RNAs are critical for the regulation of cell phenotypes in CHO cells. However, the notion that individual miRNAs usually control the expression of hundreds of different genes makes miRNA target identification highly complex. We have recently reported that the entire miR-30 family enhances recombinant protein production in CHO cells. To better understand the pro-productive effects of this miRNA family, we set out to identify their downstream target genes in CHO cells. Computational target prediction combined with a comprehensive functional validation enabled the discovery of a set of twenty putative target genes for all productivity enhancing miR-30 family members. We demonstrate that all miR-30 isoforms contribute to the regulation of the ubiquitin pathway in CHO cells by directly targeting the ubiquitin E3 ligase S-phase kinase-associated protein 2 (Skp2). Finally, we provide several lines of evidence that miR-30-mediated modulation of the ubiquitin pathway may enhance recombinant protein expression in CHO cells. In summary, this study supports the importance of non-coding RNAs, especially of miRNAs, in the context of cell line engineering.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 212, 20 October 2015, Pages 32-43
Journal: Journal of Biotechnology - Volume 212, 20 October 2015, Pages 32-43
نویسندگان
Simon Fischer, Sven Mathias, Simone Schaz, Verena Vanessa Emmerling, Theresa Buck, Michael Kleemann, Matthias Hackl, Johannes Grillari, Armaz Aschrafi, René Handrick, Kerstin Otte,