A two-stage enzymatic ethanol-based biodiesel production in a packed bed reactor

► First study of an enzymatic route for making 'in-spec' ethanol-based biodiesel. ► A solvent-free two-stage enzymatic biodiesel process in a packed bed reactor system. ► Identification of key factors for achieving efficient continuous processing in PBR. ► Evaluation of strategies for scaling-up of the studied process.