A new cloning system based on the OprI lipoprotein for the production of recombinant bacterial cell wall-derived immunogenic formulations

► We propose a new system for expression of antigens fused with the OprI lipoprotein. ► It offers tight control of expression and metal affinity chromatography purification. ► The OprI expressed by the system is triacylated, mainly by palmitic acid residues. ► The cloned antigens can be obtained in three bacterial cell wall-derived formulations. ► Applications include studies on immunization and on modulation of acquired immunity.