کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6494843 | 1418372 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rosa hybrida orcinol O-methyl transferase-mediated production of pterostilbene in metabolically engineered grapevine cell cultures
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کلمات کلیدی
MRMO-MethyltransferasePiceatannolOMTHC-ProMbCDPMSFMethylated β-cyclodextrinsPVPPSAMTCAPVDFSTSGFPhaemagglutinin - hemagglutininEDTA - اتیلن دی آمین تترا استیک اسید Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدtrichloracetic acid - اسید تریکلراکتیکtrans - ترانسResveratrol - رسوراترولStilbene synthase - سیتستین استیلبنPhenylmethanesulfonyl fluoride - فنیل متیل سولفونیل فلورایدMethyl jasmonate - متیل جاسموناتMetabolic engineering - مهندسی متابولیکMeJA - میاmultiple reaction monitoring - نظارت چندگانه چندگانهfresh weight - وزن ترPterostilbene - پتروستیلبنgreen fluorescent protein - پروتئین فلورسنت سبزPolyvinylidene fluoride - پلی وینیلیدین فلورایدpolyvinylpolypyrrolidone - پی ویوی پلیپیرلیدونVitis vinifera - گیاه انگور، مو
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Stilbenes are naturally scarce high-added-value plant compounds with chemopreventive, pharmacological and cosmetic properties. Bioproduction strategies include engineering the metabolisms of bacterial, fungal and plant cell systems. Strikingly, one of the most effective strategies consists in the elicitation of wild grapevine cell cultures, which leads to vast stilbene resveratrol accumulation in the extracellular medium. The combination of both cell culture elicitation and metabolic engineering strategies to produce resveratrol analogs proved more efficient for the hydroxylated derivative piceatannol than for the dimethylated derivative pterostilbene, for which human hydroxylase HsCYP1B1- and grapevine O-methyltransferase VvROMT-transformed cell cultures were respectively used. Rose orcinol O-methyltransferase (OOMT) displays enzymatic properties, which makes it an appealing candidate to substitute VvROMT in the combined strategy to enhance the pterostilbene production level by engineered grapevine cells upon elicitation. Here we cloned a Rosa hybrida OOMT gene, and created a genetic construction suitable for Agrobacterium-mediated plant transformation. OOMT's ability to catalyze the conversion of resveratrol into pterostilbene was first assessed in vitro using protein extracts of agroinfiltrated N. benthamiana leaves and transformed grapevine callus. The grapevine cell cultures transformed with RhOOMT produced about 16â¯mg/L culture of pterostilbene and reached an extracellular distribution of up to 34% of total production at the best, which is by far the highest production reported to date in a plant system. A bonus large resveratrol production of ca. 1500-3000â¯mg/L was simultaneously obtained. Our results demonstrate a viable successful metabolic engineering strategy to produce pterostilbene, a resveratrol analog with enhanced pharmacological properties.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: New Biotechnology - Volume 42, 25 May 2018, Pages 62-70
Journal: New Biotechnology - Volume 42, 25 May 2018, Pages 62-70
نویسندگان
Ascensión MartÃnez-Márquez, Jaime A. Morante-Carriel, Javier Palazon, Roque Bru-MartÃnez,