کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6553188 | 1422142 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inter-laboratory validation study of the ForenSeq⢠DNA Signature Prep Kit
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
The implementation of massively parallel sequencing (MPS) in forensic science revealed the advantages of the new method compared to the forensic benchmark in DNA-STR analysis, the capillary-electrophoresis (CE): Sequence information and the possibility to multiplex hundreds of markers in one multiplex PCR increase the discrimination power of a forensic (STR-) profile. The EU funded project DNASeqEx (DNA-STR Massive Sequencing & International Information Exchange) aims to evaluate MPS-based materials in their respective developmental stages using the two established platforms MiSeq FGx (Illumina) and Ion S5⢠(Thermo Fisher Scientific). As part of this project, we present here an inter-laboratory validation of the Forenseq⢠DNA Signature Prep Kit, focussing on STRs included in primer mix A. Our study comprises tests of concordance, reproducibility, sensitivity (1â¯ng, 500â¯pg, 250â¯pg, 125â¯pg, 63â¯pg, 31â¯pg) and mixtures (male-male and male-female at ratios of 1:1, 1:5, 1:10, 1:15, 1:20, 1:100, 1:500, 1:1000). Sequencing results found to be virtually concordant to CE results, to reference profiles and reproducible between duplicates and between both laboratories. We observed first locus drop-outs (LDO) at a DNA input of 63â¯pg (20 sample pool) and 125â¯pg (38 sample pool). Alleles were found to be well balanced at a DNA input of 250â¯pg or more. We found the kit to perform well on moderate mixtures (1:1-1:20).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Forensic Science International: Genetics - Volume 36, September 2018, Pages 77-85
Journal: Forensic Science International: Genetics - Volume 36, September 2018, Pages 77-85
نویسندگان
Steffi Köcher, Petra Müller, Burkhard Berger, Martin Bodner, Walther Parson, Lutz Roewer, Sascha Willuweit, The DNASeqEx Consortium The DNASeqEx Consortium,