کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
69482 48772 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Kinetics of enzymatic esterification of glycerol and free fatty acids in crude Jatropha oil by immobilized lipase from Rhizomucor miehei
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی کاتالیزور
پیش نمایش صفحه اول مقاله
Kinetics of enzymatic esterification of glycerol and free fatty acids in crude Jatropha oil by immobilized lipase from Rhizomucor miehei
چکیده انگلیسی


• Kinetics of enzymatic neutralization of Jatropha crude oil was studied.
• Esterification reaction follows a Ping Pong Bi Bi mechanism.
• Kinetic parameters indicate lipase inhibition by glycerol.
• Free fatty acids do not affect lipase activity within the ranges investigated.

Enzymatic neutralization is a recent research focus due to an increasing awareness of environmental problems caused by conventional oil refining. This study investigated the kinetics of enzymatic neutralization in crude Jatropha oil utilizing an immobilized lipase from Rhizomucor miehei. Free fatty acids, in particular oleic acid were esterified with glycerol. The reaction seems to follow a multisubstrate Ping Pong mechanism with competitive inhibition by the acyl acceptors (mono-, diacylglycerides and glycerol). Free fatty acid content did not affect lipase activity within the ranges investigated. The kinetic parameters were determined and showed that enzyme affinity is much higher to glycerol than to free fatty acids. These observations were supported by the fact that the optimum glycerol level is rather small while the reaction rate increases with increasing free fatty acid concentration.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 107, September 2014, Pages 1–7
نویسندگان
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