کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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70126 | 48812 | 2012 | 7 صفحه PDF | دانلود رایگان |

Enzymatic modification can be used to enhance the bioactive properties of phenolic compounds. The present study employed laccase from Trametes pubescens to catalyze the modification of ferulic acid in a monophasic or biphasic system, as a way of enhancing its antioxidant capacity. Two dimeric products (m/z 385.1) were purified and characterized as the β-5 and β–β dimers. In the monophasic system, the β-5 dimer was preferentially formed in dioxane while the β–β dimer formation was enriched in ethanol as co-solvent. In the biphasic system, formation of the dimers increased as the concentration of ethyl acetate was increased from 80% to 95%. The β-5 dimer showed higher antioxidant capacity than the substrate as demonstrated by standard antioxidant assays (DPPH and TEAC). These results demonstrate that alteration of reaction conditions influences the laccase-mediated oxidation of ferulic acid to form dimers with higher antioxidant capacity than the substrate.
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► Laccase-catalyzed oxidation of ferulic acid yields β-5 and β–β dimers.
► Product formation is influenced by the nature of co-solvent used.
► The β-5 dimer has higher antioxidant capacity than ferulic acid.
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 74, Issues 1–2, January 2012, Pages 29–35