کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
70139 48812 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biocatalytic activity of immobilized pointed gourd (Trichosanthes dioica) peroxidase–concanavalin A complex on calcium alginate pectin gel
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی کاتالیزور
پیش نمایش صفحه اول مقاله
Biocatalytic activity of immobilized pointed gourd (Trichosanthes dioica) peroxidase–concanavalin A complex on calcium alginate pectin gel
چکیده انگلیسی

Salt fractionated pointed gourd (Trichosanthes dioica) peroxidase–concanavalin A (PGP–Con A) complex expressed 79% of original peroxidase activity which decreased on entrapment into calcium alginate–pectin gel. Immobilized PGP–Con A complex retained 82.7% activity even at 60 °C which was achieved at pH 4.0. Urea treatment resulted in activity loss by ∼40%. With dioxane, immobilized PGP exhibited an activity of over 55% whereas an increasing concentration of dimethylformide resulted in decline. Activity of immobilized PGP–Con A was dependent on nature and concentration of detergent. Michaelis–Menten constant (Km) and Vmax for entrapped PGP–Con A complex was 0.08 mM and 15.7 mM/min, respectively.This study shows the efficacy, durability and sustainability of immobilized catalytic system which could be efficiently used for the removal of synthetic dyes from industrial effluents.

Figure optionsDownload as PowerPoint slideHighlights
► PGP proteins were immobilized with Con A and entrapped on to calcium alginate pectin gel and assessed for catalytic activity.
► Peroxidase activity of soluble and immobilized PGP was examined under the influence of several denaturants.
► Km and Vmax for entrapped PGP–Con A complex was 0.083 mM and 15.7 mM/min, respectively.
► Immobilized PGP preparations could be exploited for developing bioreactors for the treatment of phenolic and organic pollutants.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 74, Issues 1–2, January 2012, Pages 125–131
نویسندگان
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