Immobilization of glucose oxidase enzyme (GOD) in large pore ordered mesoporous cage-like FDU-1 silica

Large pore ordered mesoporous silica FDU-1 with three-dimensional (3D) face-centered cubic, Fm3m arrangement of mesopores, was synthesized under strong acid media using B-50-6600 poly(ethylene oxide)–poly(butylene oxide)–poly(ethylene oxide) triblock copolymer (EO39BO47EO39), tetraethyl orthosilicate (TEOS) and trimethyl-benzene (TMB). Large pore FDU-1 silica was obtained by using the following gel composition 1TEOS:0.00735B50-6600:0.00735TMB:6HCl:155H2O. The pristine material exhibited a BET specific surface area of 684 m2 g−1, total pore volume of 0.89 cm3 g−1, external surface area of 49 m2 g−1 and microporous volume of 0.09 cm3 g−1. The enzyme activity was determined by the Flow Injection Analysis-Chemiluminescence (FIA-CL) method. For GOD immobilized on the FDU-1 silica, GOD supernatant and GOD solution, the FIA-CL results were 9.0, 18.6 and 34.0 U, respectively. The value obtained for the activity of the GOD solution with FIA-CL method is in agreement with the 35 U, obtained by spectrophotometry.

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► The synthesis of large pore FDU-1 silica using TMB is reported for the first time.
► Large pore FDU-1 silica successfully immobilized glucose oxidase enzyme.
► FIA-CL was proposed to measure the enzymatic activity instead of spectrophotometry.