کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
70673 48841 2011 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Esterification activity and stability of Talaromyces thermophilus lipase immobilized onto chitosan
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی کاتالیزور
پیش نمایش صفحه اول مقاله
Esterification activity and stability of Talaromyces thermophilus lipase immobilized onto chitosan
چکیده انگلیسی

The Talaromyces thermophilus lipase (TTL) was immobilized by different methods namely adsorption, ionic binding and covalent coupling, using various carriers. Chitosan, pre-treated with glutaraldehyde, was selected as the most suitable support material preserving the catalytic activity almost intact and offering maximum immobilization capacity (76% and 91%, respectively). The chitosan-immobilized lipase could be reputably used for ten cycles with more than 80% of its initial hydrolytic activity. Shift in the optimal temperature from 50 to 60 °C and in the pH from 9.5 to 10, were observed for the immobilized lipase when compared to the free enzyme.The catalytic esterification of oleic acid with 1-butanol has been carried out using hexane as organic solvent. A high performance synthesis of 1-butyl oleate was obtained (95% of conversion yield) at 60 °C with a molar ratio of 1:1 oleic acid to butanol and using 100 U (0.2 g) of immobilized lipase. The esterification product is analysed by GC/MS to confirm the conversion percentage calculated by titration.

Figure optionsDownload as PowerPoint slideResearch highlights▶ Chitosan, pre-treated with glutaraldehyde, was selected as the best support material for Talaromyces thermophilus lipase (TTL). ▶ A shift in the optimal temperature from 50 to 60 °C and in the pH from 9.5 to 10, were observed for the immobilized lipase when compared to the free enzyme. ▶ A high performance synthesis of 1-butyl oleate (analysed by GC/MS) was obtained at 95% of conversion yield.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 68, Issues 3–4, March 2011, Pages 230–239
نویسندگان
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