کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
70728 | 48843 | 2009 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Screening and reaction engineering for the bioreduction of ethyl benzoylacetate and its analogues Screening and reaction engineering for the bioreduction of ethyl benzoylacetate and its analogues](/preview/png/70728.png)
Microbial reduction of benzoylacetates is already an established part of the synthetic toolbox to obtain chiral ethyl 3-hydroxy-3-phenylpropionate although bioreduction yields are low to moderate. A 30% increase in the enantioselectivity to 99% ee and a significant improvement in the yields to around 85% were achieved by combining simple screening procedures and a reaction engineering strategy. Three experimental parameters were selected for investigation: the influences of glucose, enzymatic inhibitor and biocatalyst immobilization. The screened yeasts Pichia kluyveri, Pichia stipitis and Candida utilis were found to give better yields and ee's for ethyl benzoylacetate 1a, p-nitrobenzoylacetate 1b and p-methoxybenzoylacetate 1c, respectively, with addition of glucose, α-chloroacetophenone as inhibitor and immobilization of the yeasts in alginate beads. Our results demonstrate that the optimized process can be implemented on a preparative scale without any loss in yield and ee.
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 56, Issue 1, January 2009, Pages 55–60