کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7082187 | 1460001 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Swollenin aids in the amorphogenesis step during the enzymatic hydrolysis of pretreated biomass
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کلمات کلیدی
PACECBMHMWSPCs8-aminonaphthalene-1,3,6-trisulfonic acid - 8-آمینون فتالین-1،3،6-تری سولفونیک اسیدBiorefinery - زیست پالایشBiofuel - سوخت های زیستیcarbohydrate binding module - ماژول اتصال کربوهیدراتAnts - مورچه، مورhemicellulose - همی سلولزHemicellulase - همی سلولزhigh molecular weight - وزن مولکولی بالا
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
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چکیده انگلیسی
A key limitation in the overall hydrolysis process is the restricted access that the hydrolytic enzymes have due to the macro-and-micro structure of cellulose and its association with hemicellulose and lignin. Previous work has shown that several non-hydrolytic proteins can disrupt cellulose structure and boost the activity of hydrolytic enzymes when purer forms of cellulose are used. In the work reported here, Swollenin primarily disrupted the hemicellulosic fraction of pretreated corn stover, resulting in the solubilisation of monomeric and oligomeric sugars. Although Swollenin showed little synergism when combined with the cellulase monocomponents exoglucanase (CEL7A) and endoglucanase (CEL5A), it showed pronounced synergism with xylanase monocomponents Xylanase GH10 and Xylanase GH11, resulting in the release of significantly more xylose (>300%). It appears that Swollenin plays a role in amorphogenesis and that its primary action is enhancing access to the hemicellulose fraction that limits or masks accessibility to the cellulose component of lignocellulosic substrates.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 142, August 2013, Pages 498-503
Journal: Bioresource Technology - Volume 142, August 2013, Pages 498-503
نویسندگان
Keith Gourlay, Jinguang Hu, Valdeir Arantes, Martina Andberg, Markku Saloheimo, Merja Penttilä, Jack Saddler,