کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
70926 | 48854 | 2008 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Immobilization of d-hydantoinase in polyaniline Immobilization of d-hydantoinase in polyaniline](/preview/png/70926.png)
Polyaniline (PANI) is a water-insoluble polymer that has been used as support for enzyme immobilization due to its desirable characteristics, such as ease of preparation, high synthesis yield, high stability to temperature and pH, and resistance to microbial attack. In this work an investigation was carried out to determine the best conditions to immobilize d-hydantoinase (E.C. 3.5.2.2) in this support. As result, a simple and fast methodology for d-hydantoinase immobilization in PANI is described. 100% of proteins were immobilized on the support in concentrations up to 2 mg solid/ml. Higher concentrations led to a lower protein percentage immobilized. After five reaction cycles about a half of d-hydantoinase initial activity was conserved.
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 55, Issues 3–4, November 2008, Pages 185–188