کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230783 | 1470951 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
First paraben substituted cyclotetraphosphazene compounds and DNA interaction analysis with a new automated biosensor
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کلمات کلیدی
THFNHSTMBHRPTLCN-hydroxysuccinimidetetramethylsilaneTMSEDCM.P.1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide - 1-اتیل 3- (3-دی متیل آمینوپروپیل) -карبیدیمید3,3′,5,5′-tetramethylbenzidine - 3،3 '، 5،5'-تترامیلیل بنزیدینDMSO - DMSOAmperometry - آمپرومتری Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدElectrochemical - الکتروشیمیاییBiosensor - بیوسنسورTetrahydrofuran - تتراهیدروفورانDNA interaction - تعامل DNADimethylsulfoxide - دیمتیل سولفواکسیدGenotoxicity - سمیت ژنتیکیMelting point - نقطه ذوبNeutravidin - نوتراویدینParabens - پارابنHorseradish peroxidase - پراکسیداز هوررادیشthin layer chromatography - کروماتوگرافی لایه نازک
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Cancer, as one of the leading causes of death in the world, is caused by malignant cell division and growth that depends on rapid DNA replication. To develop anti-cancer drugs this feature of cancer could be exploited by utilizing DNA-damaging molecules. To achieve this, the paraben substituted cyclotetraphosphazene compounds have been synthesized for the first time and their effect on DNA (genotoxicity) has been investigated. The conventional genotoxicity testing methods are laborious, take time and are expensive. Biosensor based assays provide an alternative to investigate this drug/compound DNA interactions. Here for the first time, a new, easy and rapid screening method has been used to investigate the DNA damage, which is based on an automated biosensor device that relies on the real-time electrochemical profiling (REPâ¢) technology. Using both the biosensor based screening method and the in vitro biological assay, the compounds 9 and 11 (propyl and benzyl substituted cyclotetraphosphazene compounds, respectively), have resulted in higher DNA damage than the others with 65% and 80% activity reduction, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 80, 15 June 2016, Pages 331-338
Journal: Biosensors and Bioelectronics - Volume 80, 15 June 2016, Pages 331-338
نویسندگان
Gönül Yenilmez Ãiftçi, Elif Åenkuytu, Saadet Elif Ä°ncir, Fatma Yuksel, Zehra Ãlçer, Tuba Yıldırım, Adem Kılıç, Yıldız UludaÄ,