Incorporación del test de dispersión de la cromatina espermática al laboratorio andrológico

Subjective evaluation of the SCD test is sufficient for the clinical laboratory, evaluating the relation halo/nucleoid for the classification. To set up the SCD a dose-response curve with oxidizing treatment is required as a positive control of damage. The use of sperm selection methods used in assisted reproduction, decreases the fragmentation index, however individual susceptibility was observed. The SCD could be delayed by freezing the sample which allows saving of reagents and processing time.