Development of a fluorescence model for the determination of constants associated with binding, quenching, and Förster resonance energy transfer efficiency

- The development of a FRET signal is modeled.
- Absorbance of acceptor and donor and KD of the complex are accounted for.
- The FRET efficiency and quenching are included as adjustable parameters.
- Software provided for fitting the parameters to the experimental data.
- Simulations can also be performed to study the impact of the various parameters.