Contactless conductivity detection for screening myrosinase substrates by capillary electrophoresis

- Myrosinase activity was assessed by CE for the first time.
- Contactless conductivity detection C4D is useful to evaluate myrosinase kinetics.
- The developed enzymatic assay is economic, simple and fast (short-end injection).
- Km value for several glucosinolates toward myrosinase were determined.
- The affinity of myrosinase toward various substrates was studied.