کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7607397 | 1493358 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparison of two low flow interfaces for measurement of mobilities and stability constants by affinity capillary electrophoresis-mass spectrometry
ترجمه فارسی عنوان
مقایسه دو رابط با جریان کم برای اندازه گیری موانع و ثابت های ثابت با استفاده از طیف سنجی الکتروفورز مویرگی
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کلمات کلیدی
ESIDB18C6BGEEOFACE18-crown-6 ether - 18 ترانس-6 اترDMSO - DMSOaffinity capillary electrophoresis - الکتروفورز مویرگی وابستهbackground electrolyte - الکترولیت پس زمینهElectroosmotic flow - جریان الکترواسموتیکDimethyl sulfoxide - دیمتیل سولفواکسیدMass spectrometry - طیف سنجی جرمیMethanol - متانولCrown ether complexes - مجتمع های پرتقالMeOH - مونHigh voltage - ولتاژ بالا، فشارقویStability constant - پایداری ثابتelectrospray ionization - یونیزاسیون الکترو اسپری
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Affinity capillary electrophoresis (ACE) is typically used for the determination of stability constant, Kst, of weak to moderately strong complexes. Sensitive detection such as mass spectrometry (MS) is required for extension of ACE methodology for estimation of Kst of stronger complexes. Consequently, an efficient interface for hyphenation of CE with MS detection is necessary. For evaluation of interfaces for electrospray ionization mass spectrometric (ESI/MS) detection in ACE conditions, potassium-crown ether complexation was used as model system. The effective mobilities of the crown ether ligands and the Kst of their potassium complexes were measured/determined by ACE-ESI/MS using two lab-made interfaces: (i) a sheathless porous tip CE-ESI/MS interface and (ii) a nano-sheath liquid flow CE-ESI/MS interface, and, in turn, compared with those obtained by ACE with UV spectrophotometric detection. Apparent stability constant of potassium-crown ether complexes in 60/40 (v/v) methanol/water mixed solvent, pH* 5.5, was about 1300âL/mol for dibenzo-18-crown-6, 1600âL/mol for benzo-18-crown-6 and 5200âL/mol for 18-crown-6 ligands, respectively. It was observed that electrophoretic mobilities from CE-MS experiments differ from reference values determined by UV detection by â¼7% depending on the CE-MS interface used. Good agreement of CE-MS and CE-UV data was achieved for nano-sheath liquid flow interface, in which the spray potential and the CE separation potential can be effectively decoupled. As for sheathless porous tip interface, a correction procedure involving a mobility marker has been proposed. It provides typically only ca. 1% difference of effective mobilities and Kst values obtained from CE-MS data as compared to those received by the reference ACE-UV method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1568, 21 September 2018, Pages 197-204
Journal: Journal of Chromatography A - Volume 1568, 21 September 2018, Pages 197-204
نویسندگان
Renáta KonáÅ¡ová, DuÅ¡an Koval, Jana Jaklová Dytrtová, Václav KaÅ¡iÄka,