کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7609354 | 1493396 | 2017 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Liquid chromatography coupled to tandem mass spectrometry for detecting ten allergens in complex and incurred foodstuffs
ترجمه فارسی عنوان
کروماتوگرافی مایع همراه با طیف سنجی جرم دو طرفه برای تشخیص ده آلرژن در مواد غذایی پیچیده و مضر
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Food allergy is a considerable heath problem, as undesirable contaminations by allergens during food production are still widespread and may be dangerous for human health. To protect the population, laboratories need to develop reliable analytical methods in order to detect allergens in various food products. Currently, a large majority of allergen-related food recalls concern bakery products. It is therefore essential to detect allergens in unprocessed and processed foodstuffs. In this study, we developed a method for detecting ten allergens in complex (chocolate, ice cream) and processed (cookie, sauce) foodstuffs, based on ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). Using a single protocol and considering a signal-to-noise ratio higher than 10 for the most abundant multiple reaction monitoring (MRM) transition, we were able to detect target allergens at 0.5Â mg/kg for milk proteins, 2.5Â mg/kg for peanut, hazelnut, pistachio, and cashew proteins, 3Â mg/kg for egg proteins, and 5Â mg/kg for soy, almond, walnut, and pecan proteins. The ability of the method to detect 10 allergens with a single protocol in complex and incurred food products makes it an attractive alternative to the ELISA method for routine laboratories.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1530, 29 December 2017, Pages 138-151
Journal: Journal of Chromatography A - Volume 1530, 29 December 2017, Pages 138-151
نویسندگان
M. Planque, T. Arnould, M. Dieu, P. Delahaut, P. Renard, N. Gillard,