کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7614209 1493621 2013 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of a multiresidue method for analysis of pesticides in sediments based on isotope dilution and liquid chromatography-electrospray-tandem mass spectrometry
ترجمه فارسی عنوان
توسعه یک روش چند منظوره برای تجزیه و تحلیل آفت کش ها در رسوب ها بر اساس رقت ایزوتوپ و کروماتوگرافی مایع - الکترو اسپری تاندیم اسپکترومتری جرمی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Because of the complexity of the sediment matrix, selective methods are necessary to identify and quantify different kinds of pesticides at a time. In this context, a multiresidue method based on isotope dilution and final analysis by liquid chromatography-electrospray-tandem mass spectrometry (LC-ESI-MS/MS) was developed for the determination of 26 pesticides and transformation products in sediment. The method developed comprises pressurized liquid extraction (PLE) and further purification of the extract by solid phase extraction (SPE) prior to analysis. In the process of method optimization various SPE cartridges as well as PLE and SPE elution solvents were evaluated. Due to the relatively high volatility of some compounds (e.g., propanil), special attention was paid to the evaporation step. Experiments comparing different pressures and times during solvent evaporation were performed with the aim to improve the recovery of these compounds. Matrix effects were also studied even though they were corrected through the use of 23 deuterated compounds as surrogate standards for quantification. The analytical method developed showed good validation parameters in terms of linearity, sensitivity (limits of detection in the pg g−1 or low ng g−1 range and limits of determination below 80 ng g−1), accuracy (relative recoveries between 92 and 118%, except for malaoxon (66.5%)), and repeatability (relative standard deviations between 1.5 and 17%, for all compounds except the acidic herbicides). Its main advantage is the simultaneous analysis of pesticides with a large variety of physical-chemical properties, as well as its improved accuracy due to the use of the isotope dilution method. Application of the method to the analysis of 5 real samples from 4 different Spanish rivers revealed the presence of 5 of the 26 target compounds, being chlorpyrifos, diuron and diazinon the most ubiquitous, as expected, due to their high bioaccumulation and low mobility features.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1305, 30 August 2013, Pages 176-187
نویسندگان
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