کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7615393 | 1493991 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Supercritical fluid extraction (SFE) of ketamine metabolites from dried urine and on-line quantification by supercritical fluid chromatography and single mass detection (on-line SFE-SFC-MS)
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کلمات کلیدی
SCO2dehydronorketamineBPRKETIPASPEDHKCyPSFELLEULOQHNK2-Propanol - 2-پروپانولLC–MS/MS - LC-MS / MSSupercritical fluid extraction - استخراج سیال فوق بحرانیSolid-phase extraction - استخراج فاز جامدLiquid-liquid extraction - استخراج مایع _ مایعValidation - اعتبار سنجیBack pressure regulator - تنظیم کننده فشار برگشتیnorketamine - تکان خوردنupper limit of quantification - حد بالای اندازه گیریlower limit of quantification - حد پایین کمیت سنجیSupercritical carbon dioxide - دیاکسید کربن فوق بحرانیCytochrome P450 - سیتوکروم پی۴۵۰Methanol - متانولMeOH - مونhydroxynorketamine - هیدروکسی نینورتامینKetamine - کتامینliquid chromatography tandem mass spectrometry - کروماتوگرافی مایع اسپکترومتری دو طرفهSupercritical fluid chromatography - کروماتوگرافی مایع فوق بحرانی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
On-line solid-phase supercritical fluid extraction (SFE) and chromatography (SFC) coupled to mass spectrometry (MS) has been evaluated for its usefulness with respect to metabolic profiling and pharmacological investigations of ketamine in humans. The aim of this study was to develop and validate a rapid, highly selective and sensitive SFE-SFC-MS method for the quantification of ketamine and its metabolites in miniature amounts in human urine excluding liquid-liquid extraction (LLE). Several conditions were optimized systematically following the requirements of the European Medicines Agency: selectivity, carry-over, calibration curve parameters (LLOQ, range and linearity), within- and between-run accuracy and precision, dilution integrity, matrix effect, and stability. The method, which required a relatively small volume of human urine (20â¯Î¼L per sample), was validated for pharmacologically and toxicologically relevant concentrations ranging from 25.0 to 1000â¯ng/mL (r2â¯>â¯0.995). The lower limit of quantification (LLOQ) for all compounds was found to be as low as 0.5â¯ng. In addition, stability of analytes during removal of water from the urine samples using different conditions (filter paper or ISOLUTE® HM-N) was studied. In conclusion, the method developed in this study can be successfully applied to studies of ketamine metabolites in humans, and may pave the way for routine application of on-line SFE-SFC-MS in clinical investigations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1076, 15 February 2018, Pages 77-83
Journal: Journal of Chromatography B - Volume 1076, 15 February 2018, Pages 77-83
نویسندگان
Robert Hofstetter, Georg M. Fassauer, Andreas Link,