کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7620012 1494477 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Non-aqueous reversed-phase liquid-chromatography of tocopherols and tocotrienols and their mass spectrometric quantification in pecan nuts
ترجمه فارسی عنوان
کروماتوگرافی مایع کروماتوگرافی تک فاز توکوفرول ها و تکت کرینوول های غیر آبی و اندازه گیری طیف سنج جرم آنها در آفتاب گردو
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
An easy and effective analytical method was developed for the simultaneous quantification of four tocopherols (Ts) and four tocotrienols (T3s) in three pecan nut cultivars (Stuart, Sioux, and Pawnee). The analytes were separated on a C30 column kept at 15 °C, under isocratic non-aqueous reversed-phase (NARP) conditions, in only 18 min and detected by atmospheric pressure chemical ionization-tandem mass spectrometry (APCI-MS/MS). The HPLC-APCI-MS/MS method was validated, according to the main FDA guidelines, and then applied for the characterization of the real samples. Analytes were extracted by cold saponification with recoveries greater than 87%. The limits of detection (LOD) and limits of quantification (LOQ) were in the range of 0.3-10 μg/100 mg and 1-30 μg/100 mg, respectively. Compared to other nuts, vitamin E composition of pecan nuts (Carya illinoinensis) has only partially been elucidated. Results have evidenced the prevalence of γ-forms for both Ts and T3 s and clear quantitative differences of the identified vitamers among the studied cultivars. The richest variety in vitamin E was Sioux with a total content of about ∼ 32 mg/100 g wet weight, followed by Stuart (∼16 mg/100 g) and Pawnee (∼9 mg/100 g).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Food Composition and Analysis - Volume 64, Part 2, December 2017, Pages 171-180
نویسندگان
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