کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7620569 1494502 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of HPLC-DAD method for determination of neonicotinoids in honey
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Development of HPLC-DAD method for determination of neonicotinoids in honey
چکیده انگلیسی
After the EU banned the use of the neonicotinoids in flowering crops that honeybees might visit, there has been an increased interest in determining the neonicotinoid residues in honeybee products such as honey. The aim of this study was to develop and optimize an HPLC-DAD analytical method with dispersive liquid-liquid microextraction (DLLME) and QuEChERS sample preparation procedures for the simultaneous analysis of seven neonicotinoids (dinotefuran, nitenpyram, thiametoxam, clothianidin, imidacloprid, acetamiprid and thiacloprid) in honey samples. The liquid chromatographic conditions were optimized by Response surface methodology with Box-Behnken design and Derringer's desirability. The optimized method was validated to fulfill the requirements of SANCO/12571/2013 standard for both sample pretreatment procedures providing results for accuracy (73.1-118.3%), repeatability (3.28-10.40%) and within-laboratory reproducibility (6.45-17.70%), limits of detection (1.5-2.5 μg kg−1) and quantification (5.0-10.0 μg kg−1) with the use of matrix-matched calibration to compensate the matrix effects. For the first time 104 honey samples from Vojvodina were analyzed. The presence of thiacloprid, imidacloprid and thiametoxam was found in a small number of samples implicating the usefulness of ongoing control of honey. Residues were confirmed by LC-MS/MS.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Food Composition and Analysis - Volume 40, June 2015, Pages 106-113
نویسندگان
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