کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7623361 | 1494544 | 2016 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isoquercitrin, isolated from Aster yomena triggers ROS-mediated apoptosis in Candida albicans
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کلمات کلیدی
NAC2',7'-dichlorodihydrofluorescein diacetateEtOAcJC-1DAPICFUH2DCFDA - H2DCFD بهN-acetylcysteine - N-استیل سیستئینROS - ROSDNA damage - آسیبDNAEthyl acetate - اتیل استاتIsoquercitrin - اسکوکریتروینTerminal deoxynucleotidyl transferase dUTP nick end labeling - ترمینال deoxynucleotidyl transferase dUTP نام نهایی پایان نامهCell cycle arrest - توقف چرخه سلولیTUNEL - تونلApoptosis - خزان یاختهایPhosphatidylserine - فسفاتیدیلسرینMethanol - متانولMeOH - مونcolony-forming unit - واحد تشکیل کلنیReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Isoquercitrin, isolated from Aster yomena triggers ROS-mediated apoptosis in Candida albicans Isoquercitrin, isolated from Aster yomena triggers ROS-mediated apoptosis in Candida albicans](/preview/png/7623361.png)
چکیده انگلیسی
Isoquercitrin has received much interest due to its various biological activities and exerts the antifungal effect. Therefore, we aimed to discover the novel antifungal mechanism of isoquercitrin in Candida albicans, showing apoptotic cell death. Using 2', 7'-dichlorodihydrofluorescein diacetate (H2DCFDA) staining, the levels of reactive oxygen species (ROS) were measured and isoquercitrin revealed different effects in a concentration-dependent manner: ROS scavenging effect (low concentration) and ROS forming effect (high concentration). Furthermore, we used the antioxidant, N-acetylcysteine (NAC) to discriminate the influence of ROS in DNA fragmentation and caspase activation. The green fluorescence of TUNEL and FITC-VADFMK was detected in high concentration-treated cells, whereas there was no fluorescence in both low concentration-treated cells and NAC-treated cells. These results demonstrated that ROS generation is the crucial factor in isoquercitrin-induced apoptosis. Thus, isoquercitrin induces ROS-mediated apoptosis in C. albicans.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Functional Foods - Volume 22, April 2016, Pages 347-357
Journal: Journal of Functional Foods - Volume 22, April 2016, Pages 347-357
نویسندگان
JiEun Yun, Eun-Rhan Woo, Dong Gun Lee,