کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7625304 | 1494558 | 2014 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
β-Mangostin induces p53-dependent G2/M cell cycle arrest and apoptosis through ROS mediated mitochondrial pathway and NfkB suppression in MCF-7 cells
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کلمات کلیدی
HCsβ-MangostinGADDXIAPPARPPMSFDCFH-DANF-kBp53MMP2′,7′-dichlorofluorescein diacetate - 2 '، 7'-dichlorofluorescein diacetateBax/Bcl-2 - Bax / Bcl-2phenylmethanesulfonylfluoride - phenylmethanesulfonyl fluorideROS - ROStumour necrosis factor alpha - تومور نکروز عامل آلفاApoptosis - خزان یاختهایTNF-α - فاکتور نکروز توموری آلفاNuclear factor-kappa B - فاکتور هسته ای-کاپا BHigh content screening - نمایش محتویات بالاMitochondrial membrane potential - پتانسیل غشای میتوکندریX-linked Inhibitor of Apoptosis Protein - پروتئین آپوپتوز وابسته به X-linkedpoly ADP ribose polymerase - پلی ADA ریبوز پلی مرازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: β-Mangostin induces p53-dependent G2/M cell cycle arrest and apoptosis through ROS mediated mitochondrial pathway and NfkB suppression in MCF-7 cells β-Mangostin induces p53-dependent G2/M cell cycle arrest and apoptosis through ROS mediated mitochondrial pathway and NfkB suppression in MCF-7 cells](/preview/png/7625304.png)
چکیده انگلیسی
β-Mangostin (βM) was isolated from Cratoxylum arborescens to investigate its anti-cancer effect in MCF-7 cells. βM induced apoptosis by down-regulation of Bcl2 and up-regulation of Bax, triggering the cytochrome c release from mitochondria to cytosol. The release of caspase-9 and -7 and consequently cleaved PARP leading to apoptotic was observed upon treatment. Reduction of both bid and caspase 8 and the up regulation of Fas showed the involvement of the extrinsic pathway. Significantly up regulated GADD45A and HRK genes were observed upon treatment, with concomitant inhibition of NF-kB to nucleus. The protein array had demonstrated the expression of HSP 70, HSP 60, XIAP, Survivin, p53 and Bax. Moreover, βM had showed p53-dependent G2/M cell cycle arrest by down regulation of cdc2 and PCNA. Together, the results demonstrated that the βM induced anti-proliferative effect, leading to G2/M phase cell cycle arrest and apoptosis through both the extrinsic and mitochondrial pathways with the involvement of the multiple pro and anti-apoptosis and NF-kB signalling pathways.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Functional Foods - Volume 6, January 2014, Pages 290-304
Journal: Journal of Functional Foods - Volume 6, January 2014, Pages 290-304
نویسندگان
Suvitha Syam, Ahmad Bustamam, Rasedee Abdullah, Mohamed Aspollah Sukari, Najihah Mohd Hashim, Mostafa Ghaderian, Mawardi Rahmani, Syam Mohan, Siddig Ibrahim Abdelwahab, Hapipah Mohd Ali,