Development and validation of automatic HS-SPME with a gas chromatography-ion trap/mass spectrometry method for analysis of volatiles in wines

► An automated headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-ion trap/mass spectrometry (GC-IT/MS) allowed the quantification of the most important volatiles in wines (simultaneously: alcohols, ester, norisoprenoids, terpenes, sequiterpens, carbonyl compounds and sulfur compounds). ► The optimal extraction of volatile compounds (optimized using Central Composite Design) occurs using a DVB/CAR/PDMS fiber, 5 ml of wine+2 g NaCl at an extraction temperature of 45 °C during 30 min. ► The method allowed the identification of a large range of volatiles compounds (64). ► The developed method can be applied to study the volatile composition of wines.