Development of a polyclonal antibody-based enzyme-linked immunosorbent assay (ELISA) for detection of Sunset Yellow FCF in food samples

► A Sunset Yellow FCF hapten was synthesized. ► The immunogen and coating antigen was prepared. ► The polyclonal antibodies were produced. ► A specific, sensitive, and simple ic-ELISA method was developed for the detection of Sunset Yellow FCF.