کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7724360 | 1497590 | 2011 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A truncated antenna mutant of Chlamydomonas reinhardtii can produce more hydrogen than the parental strain
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
الکتروشیمی
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![عکس صفحه اول مقاله: A truncated antenna mutant of Chlamydomonas reinhardtii can produce more hydrogen than the parental strain A truncated antenna mutant of Chlamydomonas reinhardtii can produce more hydrogen than the parental strain](/preview/png/7724360.png)
چکیده انگلیسی
Photoproduction of H2 gas was examined in the Chlamydomonas reinhardtii tla1 strain, CC-4169, containing a truncated light-harvesting antenna, along with its parental CC-425 strain. Although enhanced photosynthetic performance of truncated antenna algae has been demonstrated previously (Polle et al. Planta 2003; 217:49-59), improved H2 photoproduction has yet to be reported. Preliminary experiments showed that sulfur-deprived, suspension cultures of the tla1 mutant could not establish anaerobiosis in a photobioreactor, and thus, could not photoproduce H2 gas under conditions typical for the sulfur-deprived wild-type cells (Kosourov et al. Biotech Bioeng 2002; 78:731-40). However, they did produce H2 gas when deprived of sulfur and phosphorus after immobilization within thin (â¼300 μm) alginate films. These films were monitored for long-term H2 photoproduction activity under light intensities ranging from 19 to 350 μE mâ2 sâ1 PAR. Both the tla1 mutant and the CC-425 parental strain produced H2 gas for over 250 h under all light conditions tested. Relative to the parental strain, the CC-4169 mutant had lower maximum specific rates of H2 production at low and medium light intensities (19 and 184 μE mâ2 sâ1), but it exhibited a 4-times higher maximum specific rate at 285 μE mâ2 sâ1 and an 8.5-times higher rate at 350 μE mâ2 sâ1 when immobilized at approximately the same cell density as the parental strain. As a result, the CC-4169 strain accumulated almost 4-times more H2 than CC-425 at 285 μE mâ2 sâ1 and over 6-times more at 350 μE mâ2 sâ1 during 250-h experiments. These results are the first demonstration that truncating light-harvesting antennae in algal cells can increase the efficiency of H2 photoproduction in mass culture at high light intensity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Hydrogen Energy - Volume 36, Issue 3, February 2011, Pages 2044-2048
Journal: International Journal of Hydrogen Energy - Volume 36, Issue 3, February 2011, Pages 2044-2048
نویسندگان
Sergey N. Kosourov, Maria L. Ghirardi, Michael Seibert,