کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7839926 | 1505861 | 2018 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enhanced chemiluminescence by Au-Ag core-shell nanoparticles: A general and practical biosensing platform for tumor marker detection
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کلمات کلیدی
HIgGEDSPDMSHRTEMECLPBSPSAHSABSA - BSAhuman serum albumin - آلبومین سرم انسانیbovine serum albumin - آلبومین سرم گاوElectrochemiluminescence - الکتروکمی لومینسانسHuman immunoglobulin G - ایمونوگلوبولین G انسانEnergy Dispersive Spectrometer - طیف سنج انرژی پراکندهFluorescence - فلورسنسphosphate buffer solution - محلول بافر فسفاتhigh resolution transmission electron microscope - میکروسکوپ الکترونی با وضوح بالاTumor marker - نشانگر تومورChemiluminescence - نورتابی شیمیایی یا کمی لومی نسانسImmunoassay - واکنش های ایمونواسیprostate-specific antigen - پادگن مخصوص پروستاتPolydimethylsiloxane - پلیمتیلسیلوکسان
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
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چکیده انگلیسی
A general and practical chemiluminescence (CL) biosensing platform for detection of tumor marker prostate-specific antigen (PSA) as a model analyte was developed for the first time based on the excellent catalytic performance of bimetallic Au-Ag core-shell nanoparticales (Au@Ag NPs) on luminol-K3Fe(CN)6 CL system. The Au@Ag NPs were synthesized by a simple and green aqueous phase successive reduction method. The prepared Au@Ag NPs with remarkable synergistic catalytic activity effect could significantly catalyze luminol-K3Fe(CN)6 CL reaction to produce a great CL enhancement. Based on this synergistic catalytic activity, a luminol-K3Fe(CN)6-Au@Ag NPs CL system combined with immunoassay was designed for the sensitive detection of PSA. In this protocol, the Au@Ag NPs were modified with anti-PSA-antibody (Ab-Au@Ag NPs) to specifically capture target molecules PSA. In the presence of PSA, a non-competitive immunoreaction occurred between Ab-Au@Ag NPs and PSA to form an immnunonanoshperes (PSA-Ab-Au@Ag NPs). The results show that the CL catalytic ability of the immnunonanospheres decreased with the PSA concentration increasing. And the quenching degree of CL signals is proportional to the logarithm of PSA concentration in the range of 0.1â¯pgâ¯mLâ1-100.0â¯ngâ¯mLâ1. Thanks to the fine performance of the Au@Ag NPs, the detection limit of the method is down to 0.047â¯pgâ¯mLâ1 (S/Nâ¯=â¯3). Moreover, the applicability of the present method was successfully applied for PSA determination in human serum samples with recoveries of 86.1-112.5%, demonstrating great promise for application in biochemical analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Luminescence - Volume 201, September 2018, Pages 163-169
Journal: Journal of Luminescence - Volume 201, September 2018, Pages 163-169
نویسندگان
Wen-Sheng Zhang, Jun-Tao Cao, Yu-Xiang Dong, Hui Wang, Shu-Hui Ma, Yan-Ming Liu,