کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7956117 | 1513829 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Plasma-treated medium tunes the inflammatory profile in murine bone marrow-derived macrophages
ترجمه فارسی عنوان
محیط تحت درمان با پلاسما، پروفایل التهابی را در ماکروفاژهای حاصل از مغز استخوان چرک می کند
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
دانش مواد (عمومی)
چکیده انگلیسی
Macrophages are essential drivers of tumor rejection as well as tumor promotion. Especially tumor-associated macrophages (TAM) phenotypically resemble tumor-supporting alternatively activated macrophages (M2). Targeting their phenotype has long been a matter of preclinical research in oncology. Cold physical plasma and plasma-treated medium has recently been recognized as a new possible interventional strategy in tumor treatment. Whereas several studies underlined this proof-of-concept in animal studies, it is not clear how plasma affects the phenotype of macrophages. To this end, we differentiated macrophage from murine bone marrow-derived cells, and exposed them to plasma-treated cell culture medium. This led to a more pronounced NOS2 expression in several macrophage subtypes, a marker typically associated with a rather pro-inflammatory, anti-tumor phenotype. When stimulated with supernatants of pancreatic cancer cells, these macrophages released significantly increased amounts of immune-stimulatory molecules in response to plasma-treated medium. This included TNFα, IL6, IL12, CCL4, and CXCL9, whereas MCP1 and CXCL1 were significantly decreased. Interestingly, baseline expression levels as well as response to plasma-treated medium were largely opposite to macrophages stimulated with tumor cell supernatants. These results call for a more differentiated view on macrophage polarization, and emphasize the immune-modulatory role that plasma-treated medium may exert in the tumor settings.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Plasma Medicine - Volume 11, September 2018, Pages 1-9
Journal: Clinical Plasma Medicine - Volume 11, September 2018, Pages 1-9
نویسندگان
Sander Bekeschus, Lukas Scherwietes, Eric Freund, Kim Rouven Liedtke, Christine Hackbarth, Thomas von Woedtke, Lars-Ivo Partecke,