کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8088135 1521821 2015 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Metabolic engineering toward enhanced LC-PUFA biosynthesis in Nannochloropsis oceanica: Overexpression of endogenous Δ12 desaturase driven by stress-inducible promoter leads to enhanced deposition of polyunsaturated fatty acids in TAG
موضوعات مرتبط
مهندسی و علوم پایه مهندسی انرژی انرژی های تجدید پذیر، توسعه پایدار و محیط زیست
پیش نمایش صفحه اول مقاله
Metabolic engineering toward enhanced LC-PUFA biosynthesis in Nannochloropsis oceanica: Overexpression of endogenous Δ12 desaturase driven by stress-inducible promoter leads to enhanced deposition of polyunsaturated fatty acids in TAG
چکیده انگلیسی
Nannochloropsis oceanica is an important source for omega-3 long-chain polyunsaturated fatty acids (LC-PUFA) such as eicosapentaenoic acid (EPA, 20:5 n − 3), and a potent candidate for biofuel production, due to its outstanding capability for rapid induction of triacylglycerol (TAG) overproduction. In contrast to membrane lipids, TAG of N. oceanica is poor in the valuable LC-PUFA, EPA. We identified, cloned and characterized a N. oceanica microsomal-like Δ12-desaturase (NoD12) mediating the committing step of LC-PUFA biosynthesis by converting oleic acid (18:1 n − 9) to linoleic acid (LA,18:2 n − 6). We generated strains of N. oceanica overexpressing NoD12 under the control of the stress-inducible endogenous lipid droplet surface protein (LDSP) promoter, resulting in robust expression under nitrogen starvation conditions. The overexpression of NoD12 significantly altered fatty acid composition of total lipids and of individual lipid classes, such as a drastic increase in 18:2 proportion in phosphatidylcholine and in TAG was observed under nitrogen starvation. Some LA was converted further toward LC-PUFA resulting in a substantial increase in arachidonic acid (20:4 n − 6) in TAG. Our data demonstrate the feasibility of metabolic engineering to increase LC-PUFA content in the biotechnologically important microalga using native genes and promoters, and provide novel insights into the regulation of LC-PUFA flux to TAG under nitrogen starvation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Algal Research - Volume 11, September 2015, Pages 387-398
نویسندگان
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