کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8259058 | 1534630 | 2016 | 57 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Tyrosine phosphorylation of RACK1 triggers cardiomyocyte hypertrophy by regulating the interaction between p300 and GATA4
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کلمات کلیدی
PEICDK9β-MHCGATA binding protein 4GATA4nkx2.5RACK1ANFT-boxBNPFog2ET-1P300NF-κBGAPDHMEF2GSTacetyltransferase - استیل ترانسفرازendothelin-1 - اندوتلین-1TAD - بلهzinc finger domain - دامنه انگشت رویtranscription activation domain - دامنه فعال سازی رونویسیβ-myosin heavy chain - زنجیره سنگین بتا-میوزینatrial natriuretic factor - فاکتور natriuretic دهلیزیmyocyte enhancer factor 2 - فاکتور افزایش دهنده myocyte 2T-box transcription factor - فاکتور رونویسی T جعبهNuclear factor-kappa B - فاکتور هسته ای-کاپا Bphosphotyrosine - فسفاتیزینphenylephrine - فنیل آفرینHistone acetyltransferase - هیستون استیل ترانسفرازCardiac hypertrophy - هیپرتروفی قلبیpolyethyleneimine - پلی اتیلنیمینB-type natriuretic peptide - پپتید نیترویوتیک B نوعHAT - کلاهcyclin dependent kinase 9 - کیناز وابسته به سیکلین 9glutathione S-transferase - گلوتاتیون S-ترانسفرازglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Tyrosine phosphorylation of RACK1 triggers cardiomyocyte hypertrophy by regulating the interaction between p300 and GATA4 Tyrosine phosphorylation of RACK1 triggers cardiomyocyte hypertrophy by regulating the interaction between p300 and GATA4](/preview/png/8259058.png)
چکیده انگلیسی
The zinc finger protein GATA4 is a transcription factor involved in cardiomyocyte hypertrophy. It forms a functional complex with the intrinsic histone acetyltransferase (HAT) p300. The HAT activity of p300 is required for the acetylation and transcriptional activity of GATA4, as well as for cardiomyocyte hypertrophy and the development of heart failure. In the present study, we have identified Receptor for Activated Protein Kinase C1 (RACK1) as a novel GATA4-binding protein using tandem affinity purification and mass spectrometry analyses. We found that exogenous RACK1 repressed phenylephrine (PE)-induced hypertrophic responses, such as myofibrillar organization, increased cell size, and hypertrophy-associated gene transcription, in cultured cardiomyocytes. RACK1 physically interacted with GATA4 and the overexpression of RACK1 reduced PE-induced formation of the p300/GATA4 complex and the acetylation and DNA binding activity of GATA4. In response to hypertrophic stimulation in cultured cardiomyocytes and in the hearts of hypertensive heart disease model rats, the tyrosine phosphorylation of RACK1 was increased, and the binding between GATA4 and RACK1 was reduced. In addition, the tyrosine phosphorylation of RACK1 was required for the disruption of the RACK1/GATA4 complex and for the formation of the p300/GATA4 complex. These findings demonstrate that RACK1 is involved in p300/GATA4-dependent hypertrophic responses in cardiomyocytes and is a promising therapeutic target for heart failure.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease - Volume 1862, Issue 9, September 2016, Pages 1544-1557
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease - Volume 1862, Issue 9, September 2016, Pages 1544-1557
نویسندگان
Hidetoshi Suzuki, Yasufumi Katanasaka, Yoichi Sunagawa, Yusuke Miyazaki, Masafumi Funamoto, Hiromichi Wada, Koji Hasegawa, Tatsuya Morimoto,