کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8260182 | 1534656 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
I-BET151 selectively regulates IL-6 production
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کلمات کلیدی
NF-κBTNFαLPSIL-6EAEBRD4IL-1βIL-10experimental autoimmune encephalomyelitis - آنسفالومیلیت خودایمنی تجربیchromatin immunoprecipitation - ایمن سازی کروماتینInterleukin 10 - اینترلوکین 10interleukin 1β - اینترلوکین 1βinterleukin-6 - اینترلوکین ۶Enzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاtumor necrosis factor-α - تومور نکروز عامل αCytokines - سیتوکین هاnuclear factor kappa B - فاکتور هسته ای کاپا Blipopolysaccharide - لیپوپلی ساکاریدMacrophage - ماکروفاژ CHiP - چیپ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Orchestration of the inflammatory response is crucial for clearing pathogens. Although the production of multiple inflammatory cytokines has been thought to be regulated by common mechanisms, recent evidence indicates that the expression of some cytokines is differentially regulated by epigenetic regulatory mechanisms. In this study, we found that IL-6 production is selectively inhibited by the BET bromodomain protein (BRD) inhibitor I-BET151 in RAW264.7 cells stimulated with lipopolysaccharide (LPS), whereas I-BET151 did not alter the production of several other cytokines (TNFα, IL-1β and IL-10) at the concentration of IBET151 used. I-BET151 prevented the binding of CBP to the promoter of IL-6, but I-BET151 did not affect acetylation, phosphorylation, nuclear translocation, or DNA binding of p65-NF-κB. In vivo, I-BET151 treatment in the experimental autoimmune encephalomyelitis mouse model of multiple sclerosis decreased the early clinical symptoms, which are thought to be dependent on cytokine production. Altogether, these data suggest that targeting epigenetic-related proteins, such as BET proteins, may provide a strategy to reduce inflammation and the severity of inflammatory diseases, such as multiple sclerosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease - Volume 1842, Issue 9, September 2014, Pages 1549-1555
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease - Volume 1842, Issue 9, September 2014, Pages 1549-1555
نویسندگان
Elyse Barrett, Shaun Brothers, Claes Wahlestedt, Eléonore Beurel,